FIGURE 4.

Hindlimb extension reflex and gait impairment tests in CTEP‐ and vehicle‐treated SOD1^G93A and WT mice. The hindlimb extension reflex (a–d) and gait impairment (e–h) tests were performed during the disease progression in CTEP‐ and vehicle‐treated SOD1^G93A or WT male and female mice, at low‐dose (2 mg·kg⁻¹ every 48 h) or high‐dose (4 mg·kg⁻¹ every 24 h) CTEP. Animals were tested 3 days a week starting on Day 80 of life. Animals were suspended by the tail, and a 0–5 scale was used to score hindlimb posture. To evaluate gait impairment, mice were allowed to move in an open field, and a 0–5 scale score was used. Data in (a) and (e) are means ± SEM of 15 low‐dose CTEP‐treated and 15 vehicle‐treated SOD1^G93A male mice and of five low‐dose CTEP‐treated and five vehicle‐treated WT male mice. Data in (b) and (f) are means ± SEM of 15 low‐dose CTEP‐treated and 11 vehicle‐treated SOD1^G93A female mice and of five low‐dose CTEP‐treated and five vehicle‐treated WT female mice. Data in (c) and (g) are means ± SEM of 14 high‐dose CTEP‐treated and 14 vehicle‐treated SOD1^G93A male mice and of five high‐dose CTEP‐treated and five vehicle‐treated WT male mice. Data in (d) and (h) are means ± SEM of 12 high‐dose CTEP‐treated and 11 vehicle‐treated SOD1^G93A female mice and of five high‐dose CTEP‐treated and five vehicle‐treated WT female mice. *P < 0.05, significantly different from vehicle‐treated SOD1^G93A mice; two‐way ANOVA followed by Tukey's post hoc test