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(a) Formation of THIQs using enzymes and O‐ or N‐methylation using methyltransferases (MTs) and an S‐adenosylmethionine (SAM) supply/S‐adenosylhomocysteine (SAH) degradation system; (b) The route to biologically relevant methylated THIQs prepared in this work via cascades with up to 7‐enzymes. Abbreviations: E. coli (Ec); methionine adenosyltransferase (MAT); methylthioadenosine nucleosidase (MTAN); L‐methionine (L‐Met); adenosine triphosphate (ATP); S‐ribosyl‐L‐homocysteine (SRH).
