Figure 2.

a) Sequences and predicted secondary structures of selected deoxyribozymes found in this study. The arrow indicates the cleavage site. Kinetic characterization of b) AL112, c) AM101, d) AN05, e) AK104 with four RNA substrates R8 (UC), R12 (Um³C), R16 (Um⁴C) and R20 (Um⁵C). b)—e): Single‐turnover kinetics plots (left). Observed rate constants k _obs (middle). Cleavage yields FC_max after 6 h incubation (right: mean of three independent experiments with error bars showing SD); representative gel images are in the supporting information). Reaction conditions: 1 μM RNA, 10 μM deoxyribozyme, 20 mM MgCl₂, 50 mM Tris‐HCl, 150 mM NaCl, pH 7.5, 37 °C.