Figure 3.

a) Schematic representation of selective RNA cleavage with AL112, AM101 and AN05. RNA substrates R12, R16 and R20 were separately labeled with 6FAM, Cy3 and Atto647N, respectively,^[15] pooled and the mixture was subjected to cleavage with each of the three modification‐specific DNAzymes. b) PAGE analysis of the experiment. Individual imaging channels for the three fluorophores are shown in black and white, and the merged image in false‐color representation. Reaction conditions: 1 μM RNA mixture, 10 μM deoxyribozyme, 20 mM MgCl₂, 50 mM Tris‐HCl, 150 mM NaCl, 37 °C.