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. 2021 Jul 29;60(35):19483–19490. doi: 10.1002/anie.202107158

Figure 3.

Figure 3

Comparison between peptide ligation on MPE and MPyE auxiliaries performed with peptide thioester 28A (orange box) or peptide selenoester 31A (blue box). UPLC analysis of aliquots from reactions with B) peptide thioester 28A after 24 h or C) peptide selenoester 31A after 1 h and 24 h. Hydrazine (2.5 %), if added, was used to cleave thioester‐linked conjugates such as 32AN, 33AN, 35AN and 36AN. Conditions: [a] 5 mM peptides, 20 mM TCEP, 200 mM Na2HPO4, 6 M Gdn‐HCl, 3 vol % PhSH, rt, pH 7.5 [b] 5 mM, 100 mM TCEP, 200 mM Na2HPO4, 6 M Gdn‐HCl, 28 mM DPDS, rt, pH 6.2.