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A) Removal of the MPyE auxiliary from ligation products 29ZX. Representative UPLC analysis of MPyE cleavage of peptide 29LN B) at t=0 h, C) after 20 h. [a] non‐peptidic material. Conditions: 2 M amine base*, 0.5 M TCEP, 0.5 M 2‐AcPyr, 5 mM MnCl2, rt, pH 8.5. *piperazine for X=G, morpholine for X≠G.
