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Synthesis of a MUC1 peptide 45 by Pro‐Ala ligation involving peptide selenoester 42 with MPyE‐peptide 43 followed by auxiliary cleavage. UPLC analysis of aliquots withdrawn A) at the start of the ligation, B) after 24 h ligation and treatment with 2.5 % hydrazine (to confirm completion of S→N acyl transfer), C) auxiliary removal from purified ligation product and D) of purified 45.
