Table 1.

Ligation yields, auxiliary cleavage yields and estimated reaction half times of MPyE induced peptide ligation.^[a]

Ligation product	Ligation t 1/2 [c]	Ligation yield after HPLC purification[a]	Peptide after MPyE cleavage	Cleavage yield after HPLC purification[b]
29AG	≈25 min	82 %	37AG	88 %
29LG	≈40 min	78 %	37LG	54 %
29AN	≈120 min	78 %	37AN	55 %
29LN	≈160 min	72 %	37LN	39 %
29AR	≈120 min	73 %	37AR	47 %
29LR	≈360 min	66 %	37LR	58 %
29AL	≈160 min	42 %	37AL	30 %
29AV	≈360 min	58 %	37AV	22 %
29LV	≈24 h	51 %[d]	37LV	n.d.

[a] Conditions: see Figure 2. [b] Conditions: see Figure 5. [c] estimated from reaction time course in Figure 2 E). [d] fraction of product determined by UPLC analysis after 24 h.