FIGURE 3.

HDAC8 regulates microglia migration and morphology dynamic change in different activation states. (a) Microglia chemotaxis toward the control medium (vehicle), EGF (100 ng/mL) or CT2A‐glioma conditioned medium (CT2A), pretreated with vehicle or PCI‐34051 10 μM for 48 h. results are expressed as fold increases in comparison with vehicle (n = 5; * < 0.05, one‐way ANOVA). (b and c) the mean (± S.E.M.) area of Iba1⁺ cells (b) and TMEM119⁺ cells (c) (as % of the tumor area) 17 days after CT2A transplantation in mice treated with PCI‐34051 40 mg/kg, as indicated. Right, representative immunofluorescences of Iba1⁺ and TMEM119⁺ cells glioma infiltrated (green) (n = 4 mice per condition, **P < 0.01, Student's t test). Scale bars, 20 μm. (d) RT‐PCR analysis of hdac8 expression in primary microglia incubated with vehicle, IL‐4 or LPS + IFN‐γ for 48 h (n = 7–4, **P < 0.01, Student's t test). For boxplots, the center line, boxes and whiskers represent the median, inner quartiles, and rest of the data distribution, respectively. (e) Primary microglia incubated with vehicle, IL‐4 or LPS + IFN‐γ for 48 h, in presence or absence of PCI‐34051 10 μM. Form factor values (calculated as indicated in methods) are shown as mean ± S.E.M (n = 5, *P < 0.05, one‐way ANOVA). Right, representative immunofluorescences of microglia stained with Iba1 (green). Scale bars, 10 μm