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. 2021 Jul 12;60(34):18680–18687. doi: 10.1002/anie.202104497

Figure 2.

Figure 2

A) Purification of WT αHL and hybrid amyloid‐αHL oligomers with a Co‐NTA column in 50 mM Tris‐HCl, pH 8.0 with 0.5 M NaCl, 250 mM imidazole and 0.38 mM DDM micelles. Lane 1: WT αHL; lane 2–3: hybrid Aβ42‐αHL and Aβ11‐42‐αHL; lane 4: hybrid hIAPP‐αHL; lane 5–7: hybrid Aβ1‐28‐αHL, Aβ1‐17‐αHL and Aβ0‐αHL, respectively. SDS‐PAGE electrophoresis was conducted at 200 V for 25 min. B) Fluorescence emission spectra of 20 μM ANS binding to 10 μM WT αHL or hybrid amyloid‐αHL oligomers at excitation wavelength 350 nm. The fluorescence was recorded at wavelengths from 400–600 nm at room temperature in 50 mM Tris‐HCl, pH 8.0 with 0.5 M NaCl, 250 mM imidazole and 0.38 mM DDM micelles. Each experiment was repeated three times independently.