| Reagent | Final concentration | Amount to add (100 × stock) |
|---|---|---|
| NaCl | 58 mM | 34 g |
| KCl | 0.67 mM | 0.5 g |
| Ca(NO3)2 | 0.34 mM | 0.8 g |
| MgSO4 | 0.83 mM | 2.06 g |
| HEPES | 10 mM | 119 g |
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