| Reagent | Final concentration | Amount to add (1L solution) |
|---|---|---|
| NaCl | 115 mM | 6.72 g |
| KCl | 4 mM | 0.30 g |
| CaCl2-2H2O | 3 mM | 0.44 g |
| MgCl2-6H2O | 3 mM | 0.61 g |
| HEPES | 5 mM | 1.19 g |
| Glycine | 0.01 mM | 0.00075 g |
| Glucose | 10 mM | 1.80 g |
| Tubocurarine | 0.1 mM | 0.068 g |
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