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. Author manuscript; available in PMC: 2021 Sep 22.
Published in final edited form as: Cold Spring Harb Protoc. 2021 Jun 1;2021(6):10.1101/pdb.prot106880. doi: 10.1101/pdb.prot106880
Reagent Final concentration Amount to add (20 ml solution)
K-gluconate 110 mM 515.4 mg
KCl 8 mM 11.9 mg
NaCl 5 mM 5.8 mg
MgCl2-6H2O 1.5 mM 6.1 mg
HEPES 20 mM 95.3 mg
EGTA 0.5 mM 3.8 mg
ATP 4 mM 44.1 mg
GTP 0.3 mM 3.1 mg