(Working) Stock solution	Volume needed	Final concentration
a) Tris-HCl buffer (1 M, pH 8.5)	1 μL	100 mM
b) MgCl2 (200 mM)	1 μL	20 mM
c) LacβSph (100 mM)	1 μL	10 mM
d) Neu5Ac (100 mM)	1.5 μL	15 mM
e) CTP (100 mM)	1.5 μL	15 mM
f) NmCSS (0.1 mg/mL)	0.5 μL	5 μg/mL
g) PmST3 (0.2 mg/mL)	1 μL	20 μg/mL
h) Add 2.5 μL of deionized water to the reaction mixture to make the total volume of 10 μL. Vortex and spin down the tube.