(Working) Stock solution	Volume needed	Final concentration
a) Tris-HCl buffer (1 M, pH 8.5)	1 μL	100 mM
b) MgCl2 (200 mM)	1 μL	20 mM
c) GM3βSph (100 mM)	1 μL	10 mM
d) Neu5Ac (100 mM)	2 μL	20 mM
e) CTP (100 mM)	2 μL	20 mM
f) NmCSS (0.1 mg/mL)	0.5 μL	5 μg/mL