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. Author manuscript; available in PMC: 2023 Sep 19.
Published in final edited form as: J Med Chem. 2021 Sep 16;64(19):14702–14714. doi: 10.1021/acs.jmedchem.1c01214

Figure 2.

Figure 2.

Inhibitors 1, 2 and remdesivir potently blocked the infectivity and cytopathic effect of SARS-CoV-2WK-521 in VeroE6 cells. VeroE6 cells were exposed to SARS-CoV-2WK-521 at an MOI of 0.05 and cultured in the presence or absence of test compounds. After 3 days, the cells were fixed with 4% paraformaldehyde and immunocytochemistry was conducted using the IgG fraction from serum of a COVID-19-convalescent patient, who had high-titer SARS-CoV-2-binding IgG,44 and a green fluorescence-conjugated goat polyclonal anti-human-IgG-Alexa Fluor 488 Fab fragment as the primary and secondary antibodies, respectively. SARS-CoV-2 antigens, filamentous actin, and nuclei are shown in green, red, and blue, respectively.