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Ethidium bromide-stained polyacrylamide gel of PCR products obtained from enzymes used for pretreatment of samples for fungal PCR and controls by using primers S1 and CUF1. Lanes: L, 100-bp ladder; 1, 100 pg of C. albicans DNA; 2, 100 pg of A. fumigatus DNA; 3, Zymolyase (Seikagaku); 4, Zymolyase (ICN); 5, lysing enzymes (Sigma); 6, lyticase (Sigma); 7, proteinase K (Amresco); 8, proteinase K (Boehringer); 9, negative control.
