TABLE 1.
Sensitive fungal PCR assays with fungi and commercial enzymes frequently used for pretreatment
| Sample | Source | Amplification product result with primer pair:
|
|
|---|---|---|---|
| S1-CUF1 | F5-F6 | ||
| C. albicans | DNA | Positive | Positive |
| A. fumigatus | DNA | Positive | Positive |
| Zymolyase-20T | Seikagaku, Tokyo, Japan | Positivea | Positivea |
| Zymolyase-20T | ICN, Aurora, Ohio | Positivea | Positivea |
| Lyticase | Sigma, St. Louis, Mo. | Negative | Negative |
| Lysing enzymes (T. harzianum) | Sigma, St. Louis, Mo. | Negative | Negative |
| Proteinase K | Amresco, Solon, Ohio | Negative | Negative |
| Proteinase K | Boehringer, Mannheim, Germany | Negative | Negative |
| Negative control | Sterile water | Negative | Negative |
a
Identified by sequencing as DNA from a member of the Saccharomyces sensu stricto complex or K. lodderae.