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. 2021 Sep 22;16(9):e0257576. doi: 10.1371/journal.pone.0257576

Fig 4. α5 integrin is required for optimal TNF-mediated pro-inflammatory response and NFκB activation.

Fig 4

(A) Medium supernatant collected from BMDMs treated with TNF (100ng/ml) for 16h in the presence of either control IgG or α5β1 blocking antibody (75μg/ml) was analyzed for CCL3 production by ELISA (n = 8; two independent experiments). (B) Cell lysate collected from WT and α5 integrin KO HAP-1 cells treated with TNF (30ng/ml) for indicated time periods were subjected to western blot analysis to detect status of IκB protein. The immunoblot is representative of three independent experiments with similar results. (C) Densitometric analysis of the western blot presented in Fig 4b. The densitometric quantification values for IκB immunoblot represent the ratio of IκB:actin and the fold-induction was calculated after normalizing with the control 0 min group. ELISA data are shown as Mean ± SEM. *p ≤ 0.05 using a Student’s t-test. The densitometric values represent the mean ± SEM from three independent experiments. The p value shown in the figure was calculated using a Student’s t-test (*p ≤ 0.05).