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. 2021 Sep 22;16(9):e0257576. doi: 10.1371/journal.pone.0257576

Fig 5. TNF induces C25H expression via type-I interferon independent mechanism.

Fig 5

(A) 2fTGH and U5A cells treated with TNF (30ng/ml) for 1h were analyzed for C25H mRNA expression by RT-qPCR (n = 5–6; two independent experiments). (B) Cell lysate collected from MEFs treated with IFN-β protein (100 IU/ml) for 30mins in the presence of either control IgG or IFNR1 blocking antibody (IFN Ab) (50μg/ml) were subjected to western blot analysis to detect status of phosphorylated STAT-1 (phospho-STAT1) protein. The immunoblot is representative of two independent experiments with similar results. (C) MEFs treated with TNF (10ng/ml) for 4h in the presence of either control IgG or IFN Ab (50μg/ml) were analyzed for C25H mRNA expression by RT-qPCR (n = 5–6; two independent experiments). RT-qPCR data are shown as Mean ± SEM by using one-way ANOVA multiple comparison test. ns; not significant.