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. 2021 Sep 8;11:745584. doi: 10.3389/fonc.2021.745584

Figure 4.

Figure 4

Ursolic acid impairs the glycolytic metabolism of breast cancer cells by activating Cav-1 signaling. (A) MCF-7 and MDA-MB-231 cells were treated with 10~20 μM ursolic acid for 48 h Cell immunofluorescence assay was conducted to investigate the effect of ursolic acid on Cav-1 expression in breast cancer cells. Scale bar=10 μm. (B) The expression change of Cav-1 protein in MCF-7 and MDA-MB-231 cells after ursolic acid (1~30 μM) treatment for 48 h (C) MCF-7 and MDA-MB-231 cells were treated with 10~20 μM ursolic acid for 24 h The caveolae structures in breast cancer cells were observed by the transmission electron microscope. (D–F) Breast cancer cells were transfected with Cav-1 specific siRNAs or treated with 10 μM ursolic acid for 48 h The combinational effects of Cav-1 knockdown and ursolic acid treatment on lactate production (D), ECARs (E) and glycolysis-related proteins (F) of breast cancer cells were detected. N = 3. * p < 0.05. ** p < 0.01.