Skip to main content
. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Curr Protoc. 2021 Sep;1(9):e249. doi: 10.1002/cpz1.249

Figure 6: Screening for developmental toxicity in zebrafish.

Figure 6:

After fertilization, zebrafish embryos are collected and placed into individual wells of a 96-well plate in embryo water with or without the biological nanoparticles to test, such as milk derived nanovesicles (MNVs). The water is replaced every 24 hours, and the embryos monitored for up to 6 days. Hatching is monitored starting from 48 hours post fertilization (hpf) until all embryos have hatched. The zebrafish are examined daily, and any mortality noted. Survivors are examined daily for visible malformations using a checklist.