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. 2021 Sep 7;13(17):20962–20991. doi: 10.18632/aging.203495

Figure 3.

Figure 3

(A) Dose-dependent effects of glutamine on TIS escape. Doxorubicin-induced senescent MCF-7 and A549 cells were grown in media with different glutamine concentrations. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of three independent experiments. (B) Dose-dependent effects of glutamine on CD44+/CD24 subpopulation. MCF-7 cells were grown for 48 hours in media with different glutamine concentrations. Expression of CD44 and CD24 was analyzed by flow cytometry. Data are mean ± S.D. of three independent experiments.