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. 2021 Sep 7;13(17):20962–20991. doi: 10.18632/aging.203495

Figure 6.

Figure 6

The effect of glutamine deprivation on TIS escape. (A) Doxorubicin-induced senescent MDA-MB-231 cells were grown in the presence or in the absence of glutamine. Colonies that evaded the senescent growth arrest were counted. Data are mean ± S.D. of three independent experiments. (B) Doxorubicin-induced senescent TS/A cells were grown in the presence or in the absence of glutamine. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of three independent experiments. (C) Doxorubicin-induced senescent ID8 cells were grown in the presence or in the absence of glutamine. A representative image of a colony escape assay in ID8 cells is shown. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of three independent experiments. (D) CisPt-induced senescent MDA-MB-231 cells were grown in the presence or in the absence of glutamine. Colonies that evaded the senescent growth arrest were counted. Data are mean ± S.D. of three independent experiments. (E) Effect of glutamine on CD44+/CD24− subpopulation. MDA-MB-231 cells were grown for 48 hours in the presence or in the absence of glutamine. Expression of CD44 and CD24 was analyzed by flow cytometry. Data are mean ± S.D. of three independent experiments. (F) GPNA treatment abolishes escape from TIS. Doxorubicin-induced senescent MDA-MB-231 cells were grown in complete medium (+Gln), in the absence or in the presence of 1 mM GPNA. Colonies that evaded the senescent growth arrest were stained and counted. Data are mean ± S.D. of two independent experiments.