Figure 2.

Expression of heat shock proteins during ARPE-19 senescence. (A) Immunoblot of HSP90α, HSP70, HSC70, BIP, HSPB1 (HSP27), HSPB5 (αB-crystallin) and β-actin in ARPE-19 cells treated with H₂O₂ in the same way as Figure 1B. (B) Densitometry quantitation of protein bands in A in image J. The data shown are mean ± SD. The two-tailed unpaired t-test was used for statistical analysis (n = 3). (C–G) Quantitative PCR to measure the expression of HSP90α, BIP, HSC70, HSPB1 and HSPB5 in the cells treated with H₂O₂ the same way as A. The data were from three independent experiments. The two-tailed unpaired t-test was used for statistical analysis (n = 3).