Table 1. The primers used for quantitative PCR.
Primer name | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
IL-1β | AGTACCTGAGCTCGCCAGT | TGGTGGTCGGAGATTCGTAG |
IL-6 | TGAACTCCTTCTCCACAAGCG | CCGTCGAGGATGTACCGAAT |
IL-8 | GCTCTGTGTGAAGGTGCAGTT | ACCCAGTTTTCCTTGGGGTC |
MCP | CGCCTCCAGCATGAAAGTCT | AGGTGACTGGGGCATTGATT |
P21 | AAGTCAGTTCCTTGTGGAGC | GCCATTAGCGCATCACAGTC |
P53 | ACCTATGGAAACTACTTCCTGAAA | CTGGCATTCTGGGAGCTTCA |
BCL-XL | CCTAAGGCGGATTTGAATAATCTT | CCAAAACACCTGCTCACTCAC |
HSPB5 | CTGAGTCCCTTCTACCTTCGG | ATCCTGGCGCTCTTCATGTT |
HSP27 | CACGCAGTCCAACGAGATCA | TTACTTGGCGGCAGTCTCAT |
HSC70 | TATTGGAGCCAGGCCTACAC | TCAGTGTCCGTAAAGGCGAC |
HSP90α | GCTCCAAGGGTTGACATGGT | TGTAACTCATGGACGCAGGG |
BIP | GAACGTCTGATTGGCGATGC | ACCACCTTGAACGGCAAGAA |
VEGFA | CCCACTGAGGAGTCCAACATC | CTGCATTCACATTTGTTGTGCTG |
C-FLIP | TGGTTCCACCTAATGTCA | GAGCAGTTCAGCCAAGTC |
ACTB | ACCGCGAGAAGATGACCCAG | GGATAGCACAGCCTGGATAGCAA |
GAPDH | GACAGTCAGCCGCATCTTCT | GCGCCCAATACGACCAAATC |
HIF1α | TCAAAGTCGGACAGCCTCAC | GATTGCCCCAGCAGTCTACA |
GLB1 | TTCGCATCCTCCCTCTGTTG | TCAAACATCCTCTGGGTGGC |