FIG. 7.

IL-1- and TPA-dependent induction of c-Jun, JunD, and ATF-2 phosphorylation. (A) IL-1- and TPA-dependent induction of c-Jun phosphorylation. Western blots were incubated with a phosphospecific polyclonal antibody, recognizing the Ser⁷³-phosphorylated, but not the unphosphorylated, c-Jun. (B) Mobility shift of JunD isoforms during IL-1- or TPA-mediated induction. Immunoblotting was performed as described for Fig. 6C. The arrows indicate the two JunD translation products (40 and 37 kDa), while the asterisks show the JunD modified isoforms. The immunoblotting specificity was verified by preincubating the antibodies with the JunD immunizing peptide. (C) IL-1- and TPA-dependent induction of ATF-2 phosphorylation. Western blots were incubated with a phosphospecific polyclonal antibody, recognizing the Thr⁷¹-phosphorylated, but not the unphosphorylated, ATF-2. Primes indicate minutes.