Fig 3.

Fitted parameters derived fromFig. 2Band Supplementary Fig. S4. (A) $k_{\mathrm{lock}}$ and (B) $K_{\mathrm{M}}^{\mathrm{I}{\mathrm{F}}_1}$. In (A) and (B), the circles and error bars in each data point represent the fitted parameter and fitting error determined in Fig. 2B and Supplementary Fig. S4. (C) The effective binding constant $k_{\mathrm{on}}^{\mathrm{ }\mathrm{eff}}\equiv k_{\mathrm{lock}}/K_{\mathrm{M}}^{\mathrm{I}{\mathrm{F}}_1}$. The gray line represents the fitting curve of Eq. 5 and the orange line represents the duty ratio of catalytic dwell against overall reaction time. The circles and error bars in each data point represent the mean value and the SD calculated from Fig. 3A and B. In Fig. 3A–C, grey and blue points represent the results for bMF₁(wild-type) at 0.1–1000 μM and bMF₁(βE188D) at 1 mM ATP, respectively. To see this figure in colour, go online.