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. 2021 Sep 22;7(39):eabi7828. doi: 10.1126/sciadv.abi7828

Fig. 5. A large portion of daily hepatic metabolism requires extrahepatic BMAL1 even in the presence of a feeding-fasting rhythm.

Fig. 5.

(A to F) Analysis of diurnal metabolite profiles under NF as in Fig. 4 (n = 4). (A) Phase-sorted heatmap of WT NF oscillating metabolites that failed to oscillate in Liver-RE NF, thereby showing a dependence on the rest of the clock network (“network-dependent”). (B) Pathways containing metabolites that oscillate in WT NF-only (JTK_CYCLE, P < 0.05), ranked by percentage of metabolites oscillating (numbers in each class are shown to the left of bars). (C) Phase-sorted heatmap showing all WT oscillating lipid metabolites. (D) Examples of WT NF-only oscillating and other informative lipid metabolites (means ± SEM, n = 4 per group, per time point; two-way ANOVA with Bonferroni post hoc tests; P < 0.05; *WT versus KO, #WT versus Liver-RE, and $KO versus Liver-RE). (E) Number of pairwise metabolite correlations under NF. (F) Overlap of metabolite correlation pairs between genotypes.