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Cytotoxic effect of AME on 4T1 cells. 4T1 cells (5 × 104 cells/mL) were treated with AME for 24 h and then subjected to MTT assay. The viable cells were then quantified using a 450-nm ELISA reader. (a) Percentage of 4T1 cells viability. The viable cells were calculated in accordance with the analysis procedure (p < 0.05). (b) Cells’ appearance after being treated with AME under an inverted microscope with a 100x magnification
