Figure 4.
Effects of AME on 4T1 cells in-vitro. (a) 4T1 cells (5 × 105 cells/mL) were treated with Annona muricata (12.5 μg/mL and 25 μg/mL) alone and in combination with dox (100 nM) for 1, 2, and 3 days and subjected to β-galactosidase staining. As a positive control, cells were treated with dox (100 nM) for 3 days. The percentages of senescent cells (β-galactosidase-positive cells) were calculated (n = 3). (b) The senescence induced cells’ appearance after being treated with AME and dox under an inverted microscope with a 100× magnification