Figure 2.

Effect of FDMPO on caspase-3 activation and apoptosis and necrosis% in melanoma cells and normal fibroblcytes.  (A) Cells (10⁶ cells/mL) were treated with 50 µM of FDMPO at 12 h. Caspase-3 activity was determined by Sigma-Aldrich kit. The kit determines produced pNA that is released from the interaction of caspase-3 and AC-DEVD-pNA (peptide substrate). FDMPO significantly increased the activity of caspae-3 in melanoma cells but not in human normal fibroblcytes. Z-IETD a caspase 8 inhibitor not affected on caspase 3 activation. Values are expressed as mean ± SD of three separate experiments (n = 5). ^***Significant difference in comparison with cancerous control (p<0.001) and ^###Significant difference in comparison with 50 µM new chalconeferrocenyl derivative (p<0.001). (B) Effects of FDMPO on apoptosis in melanoma cells and normal fibroblcytes. FDMPO-induced apoptosis in melanoma cell but not in normal fibroblcytes at IC50 concentrations (50 μM) within 12 h. Assays were performed in triplicate (n = 3).