Table 1. Comparative table of Sanger/MiSeq/Nanopore sequencing platforms for DNA species identification summarizing approximate platform cost; running cost; analysis time; data output; accuracy and key benefits/limitations.
| Platform | Platform cost | Running costs (per mb) | Analysis time | Data types | Read length | Output | Accuracy (consensus) | Key benefits and limitations | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Wet laboratory | Run time | Dry laboratory | ||||||||
| Sanger | $250 000 | $500 | 8 h | 20 min–3 h | 5–10 min/per sample | ab1 | <1000 bp | 1 read per sequencing reaction | 99.999% | Low throughput High accuracy |
| Illumina MiSeq | $125 000 | $0.5 | 8–24 h | 21–56 h | ∼15 min/1–50 samples | fastq | 2 × 300 bp | 15 Gb (∼25 M reads) | 99.99% | High-throughput Good accuracy Short reads |
| ONT MinION | $1000 | $1.9–$9 | 8–10 h | 1–48 h | ∼15 min/1–50 samples | fast5, fastq | 200–∼1 mb | 1–44 Gb | >99% | Affordable Lower accuracy Long reads |