(A-C) Tumor growth analysis in C57BL/6J mice with
B16–F0 cells with overexpression of wild type AGGF1 (WT vs. control Ctrl)
and six AGGF1 somatic mutations in the FHA domain. (A) Images of
tumors excised from mice at the end of the study (day 18). (B)
Tumor growth as measured by the tumor volume at different time points.
(C) Tumor weight at the end of the study. B16–F0 cells
transfected with an expression plasmid for FLAG-tagged wild type AGGF1, mutant
AGGF1 without the FHA domain (del-FHA) or empty vector control pcDNA3.1 (CTRL).
**P < 0.01, n = 5–6/group.
(D) Western blot analysis showing the effects of six AGGF1
mutations in the FHA domain on the expression levels of p53 and p21. GAPDH was
sued as loading control. Quantifications were normalized to GAPDH and show as
relative density (bottom).
(E) Representative images for tumor cell invasion assays
using HCT116 cells transfected with an expression plasmid for wild type AGGF1,
mutant AGGF1 with the FHA domain deleted or empty vector control (Ctrl).
Quantification of invasion cells was shown (right).