| Cell line (Homo sapiens) |
HEK293-E |
Yves Durocher, PMID:11788735
|
RRID:CVCL_6974
|
|
| Transfected construct (Homo sapiens) |
pCMV6-CD209 |
Origene |
Cat.# SC304915 |
Plasmid for CD209 cDNA expression in cell-based binding assay |
| Transfected construct (Homo sapiens) |
pTT3-ACE2-BLH |
PMID:33432067
|
|
Plasmid for recombinant ACE2 extracellular domain, for plate-based assays as the immobilised form |
| Transfected construct (Homo sapiens) |
pTT3-CD209-BLH |
This paper |
|
Plasmid for recombinant CD209 extracellular domain for plate-based assays as the immobilised form |
| Transfected construct (Homo sapiens) |
pTT3-Cd4d3+ d4 |
Addgene |
RRID:Addgene_32402
|
Plasmid for recombinant tag control (Cd4 domains 3 and 4) |
| Transfected construct (Homo sapiens) |
pTT3-SPIKE-COMP-BLac |
This paper |
|
Plasmid for recombinant SARS-CoV-2 spike extracellular domain for plate-based assays as the soluble form |
| Transfected construct (Homo sapiens) |
pTT3-BirA-FLAG |
Addgene |
RRID:Addgene_64395
|
Biotin ligase plasmid for recombinant protein biotinylation |
| Peptide, recombinant protein |
Streptavidin R-phycoerythrin |
BioLegend |
Cat.# 405245 |
For tetramer staining in cell-based binding assay |
| Chemical compound, drug |
DAPI (4',6-diamidino-2-phenylindole) |
BioLegend |
Cat.# 422801 |
1 μM for flow cytometry live/dead staining |
| Chemical compound, drug |
D-biotin |
Sigma-Aldrich |
Cat.# 2031 |
100 μM supplemented to cell culture media for biotinylation |
| Software, algorithm |
R (version 4.0.3) |
R Foundation |
www.r-project.orgRRID:SCR_001905
|
Analysis and generating plots |
