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. 2021 Sep 18;14:4723–4741. doi: 10.2147/JIR.S323435

Figure 3.

Figure 3

Recombinant exogenous WNT16 inhibits osteoclastogenesis in primary calvarial bone cell cultures and in spleen cell cultures. Primary calvarial bone cells were stimulated with mOSM (30 ng/mL) in the presence or absence of WNT16 (1 µg/mL) for 9 days. Cells were TRAP stained (A) and number of TRAP+MuOCL with more than 3 nuclei (B) was analyzed. Scale bars, 500 µm. Gene expression of Acp5 (C), Calcr (D), Tnfs11 (E) and Tnfrs11b (F). Primary spleen cell cultures were stimulated with 4 ng/mL RANKL in the presence or absence of 1 µg/mL WNT16 for 4 days. Cells were TRAP stained (G) and number of TRAP+MuOCL with more than 3 nuclei (H) was analyzed. Scale bars, 200 µm. Gene expression of Acp5 (I) and Calcr (J). Individual values are presented in all graphs with the mean shown as horizontal lines and ±SEM as vertical lines. ***P<0.001, *P < 0.05 vs mOSM or mRANKL-treated cells, Student’s t-test.