Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 18;14:4723–4741. doi: 10.2147/JIR.S323435

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Henning et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Osteoclastogenesis stimulated by OSM is enhanced in primary calvarial bone cell cultures from Wnt16^−/- mice. Primary calvarial periosteal cells from wild-type (WT) and Wnt16^−/- mice were stimulated with 100 ng/mL mOSM for 11 days. Cells were TRAP stained (A) and numbers of TRAP⁺MuOCL with more than 3 (B) and 10 nuclei (C) as well as the total surface (D) covered by TRAP⁺MuOCL were analyzed. Scale bars, 500 µm. Expression of Acp5 (E), Ctsk (F), Tnfs11 (G) and Tnfrs11b (H) after mOSM treatment for 11 days. Individual values are presented in all graphs with the mean shown as horizontal lines and ±SEM as vertical lines. ^###P<0.001, ^##P < 0.01 vs WT, Student’s t-test. ***P<0.001*P < 0.05 vs genotype specific untreated control, two-way ANOVA followed by Sidak’s multiple comparison test for the effect of mOSM treatment. ^§§§P<0.001, ns (not significant) P>0.05 for the effect by mOSM in WT vs the effect by mOSM in Wnt16^−/- cells analyzed by interaction in two-way ANOVA.