Table 2.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal	Fig. 1A
Phenotype	Qualitative analysis	Positive for Oct4, Nanog, Sox2	Fig. 1B
	Quantitative analysis	SCVIi003 (Oct4-99%; Nanog-80.1 ± 8.1%; Sox2-99%) SCVIi004 (Oct4-99%; Nanog-82.6 ± 6.4%; Sox2-99%) SCVIi005 (Oct4-99%; Nanog-80.1 ± 3.4%; Sox2-99%)	Supplementary Fig. 1
Genotype	Whole genome array (KaryoStat™ Assay) Resolution 1–2 Mb	Normal karyotype: 46, XY for SCVIi003-A and XX for SCVIi004-A and SCVIi005-A	Supplementary Fig. 2
Identity	Microsatellite PCR (mPCR) OR STR analysis	N/A 24 markers tested with 100% match	N/A Not shown but available with author
Mutation analysis	Sequencing	Heterozygous Heterozygous Heterozygous	Fig. 1D
	Southern Blot or WGS	N/A	N/A
Microbiology and virology	Mycoplasma	Negative	Supplementary Fig. 3
Differentiation potential	Trilineage in vitro differentiation by immunofluorescence analysis	Positive staining of three germ layer markers: ectodermal (OTX2), mesoderm (BRACHYURY), endoderm (SOX17)	Fig. 1C
Donor screening	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info	Blood group genotyping	N/A	Not shown but available with author
	HLA tissue typing	N/A	Not shown but available with author