Table 3:
Reagents details.
| Antibodies used for immunocytochemistry/flow-cytometry | |||
|---|---|---|---|
|
| |||
| Antibody | Dilution | Company Cat # and RRID | |
|
| |||
| Pluri potency Marker | Mouse IgG2bκ Anti-OCT-3/4 | 1:200 | Santa Cruz Biotechnology Cat# sc-5279, RRID: AB_628051 |
| Pluri potency Marker | Rabbit Anti-NANOG | 1:200 | Proteintech Cat# 142951-1-AP, RRID: AB_1607719 |
| Pluripotency Marker | Mouse IgG1κ Anti-SOX 2 | 1:200 | Santa Cruz Biotechnology Cat# sc-365823, RRID: AB_10842165 |
| Ectoderm Marker | Goat Anti-OTX 2 | 1:200 | R&D Systems Cat# 963273, RRID: AB_2157172 |
| Endoderm Marker | Goat Anti-SOX 17 | 1:200 | R&D Systems Cat# 963121, RRID: AB_355060 |
| Mesoderm Marker | Goat Anti-BRACHYURY | 1:200 | R&D Systems Cat# 963427, RRID: AB_2200235 |
| Mesoderm Marker | Mouse Anti-Smooth Muscle Actin | 1:200 | R&D Systems Cat# MAB1420, RRID: AB_262054 |
| Secondary Antibody | Alexa Fluor 488 Goat Anti-Mouse IgG1 | 1:1000 | Thermo Fisher Scientific #A-21121 RRID: AB_2535764 |
| Secondary Antibody | Alexa Fluor 647 Goat Anti-Mouse IgG2b | 1:250 | Thermo Fisher Scientific #A-21242 RRID: AB_2535811 |
| Secondary Antibody | Alexa Fluor 555 Goat Anti-Rabbit IgG (H + L) | 1:500 | Thermo Fisher Scientific #A-21428 RRID: AB_141784 |
| Secondary Antibody | Alexa Fluor 488 Donkey Anti-Goat IgG (H + L) | 1:1000 | Thermo Fisher Scientific #A-11055 RRID: AB_2534102 |
| Primers | |||
|
| |||
| Target | Forward/Reverse primer (5′-3′) | ||
|
| |||
| Genotyping | PKP2 (c.2065_2070delinsG) | F: AGGCCGGTTTATCACCTACT R: TCTTCATCAACCTCTGGTAATC |
|
| Genotyping | PKP2 (c.235C>T) | F: TGACGAGAAGTTTGCCCCTT R: ACGTTTAATACTTGTTCTTGGCCT |
|
| Genotyping | PKP2 (c.1725_1728dup) | F: AACCTAAAACCAAGCGGCTAT R: TCCAAACACCTGGAAGAAGCA |
|