Figure 7.

Olaparib protected mitochondrial function in Caco-2 monolayers subjected to H₂O₂-induced oxidative stress. Caco-2 monolayers were pretreated with 10 μM olaparib for 30 minutes and then treated with 1000 μM H₂O₂ for 2 hours. The CTRL and H₂O₂ groups received the same amount of DMSO as the olaparib-treated cells. Seahorse XFp Mito Stress test was performed, when oligomycin (1 μM), FCCP (1 μM), and the mixture of rotenone and antimycin A (1 μM) were injected sequentially. (a) Key parameters of mitochondrial respiration measured by Seahorse XFp Extracellular Flux Analyzer. (b) Measurement of oxygen consumption rate (OCR). Data combined from 2 separate experiments are expressed as mean ± SD (n = 4). Bioenergetic parameters: (c) basal respiration, (d) ATP production, (e) coupling efficiency, (f) maximal respiration, (g) spare respiratory capacity, and (h) proton leak were calculated. Results are expressed in percentage of control (mean ± SD of two independent experiments, n = 4); ns: not significant; ^∗∗P < 0.01, ^∗∗∗P < 0.001.