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. 2021 Sep 18;15:3965–3978. doi: 10.2147/DDDT.S332847

Figure 4.

Figure 4

LncPVT1 regulates GPX4 expression via suppressing miR-214-3p. (A and B) Tumor (n=36) and non-tumor (n=36) tissues were collected, then levels of lncPVT1, GPX4, and miR-214-3p were detected by RT-PCR (A). Their correlation was analyzed by Pearson test (B). (C) Binding sites of miR-214-3p on lncPVT1 and 3ʹUTR of GPX4 predicted by ENCORI. (D) RT-PCR to determine miR-214-3p level in liver cancer cells under transfection of miR-214-3p mimics. (E and F) Luciferase activity of wild type (WT) and mutated type (MUT) lncPVT1 reporter gene vector. (G) RNA pulldown assay to detect enrichment of lncPVT1 by miR-214-3p. Bio-NC, biotin-labeled negative control mimics; Bio-miR-WT, biotin-labeled wild-type miR-214-3p mimics; Bio-miR-MUT, biotin-labeled mutated miR-214-3p mimics. (H) RT-PCR to determine miR-214-3p level. (I) Luciferase activity of wild type (WT) and mutated type (MUT) GPX4 3ʹUTR reporter gene vector. (J) Level of GPX4 detected by RT-PCR and Western blot analysis. **p < 0.01.