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. 2020 Sep 3;70(10):1904–1913. doi: 10.1136/gutjnl-2020-321016

Figure 5.

Figure 5

Multiplex immunofluorescence validates cell type differences across PanNET subtypes. (A) Representative multiple immunofluorescence images demonstrating protein expression of CD68 (macrophages), FOXP3 (Tregs), CD8 (cytotoxic T cells), CD20 (B cells) and pan-CK (cancer cells) in MLP-1 (left) and intermediate (right) samples. Nuclei are stained with DAPI. Bars represent ×20 magnification. (B) The median score of CD68 immunofluorescence staining (cell type/megapixel) computed from multiple regions of multiple slides for 30 PanNET samples and plotted for subtypes. (C) Gene expression levels of CD68 across PanNET subtypes in validation cohort 1. CK, cytokeratin; DAPI, 4′,6-diamidino-2-phenylindole; FDR computed by Kruskal-Wallis test after multiple testing corrections. FDR, false discovery rate; MLP, metastasis-like primary; Treg, T-regulatory cell.