Fig. 2.

The integrated microfluidic chip. (A) (Left) Schematic of the microfluidic chip layout, which consists of a lysis chamber, a mixing chamber, reaction chambers, passive valves, and wax valves. (Right) A enlarged view of the reaction chambers and the wax valves with the information of dimensions. (B) A digital image of the fabricated microfluidic chip. (C) The fluorescence images of the reaction chambers before and after the RT-LAMP reaction. Chamber C2, C3, C4 were pre-dried with the SARS-CoV-2 specific primers, while Chamber C1 is a negative control. (D) The real-time fluorescence signal is transmitted and displayed on a smartphone together with the temperature profiles of each heater.