Figure 1: Presence of ApoM enhances binding binding, association and transendothelial transport of HDL in bovine aortic endothelial cells (BAECs).

BAECs were cultured for 72 hours before they were incubated for 1 hour with 10μg/mL of ¹²⁵I-HDL from wild type mice or apom knock-out mice (A- C) or radioioidinated total HDL or radioioidinated apoM-depleted HDL from humans at indicated amounts (D-F) in the absence (total) or in the presence of 40-fold excess of unlabeled HDL, to record nonspecific interactions. Specific binding, association and transport were calculated by subtracting nonspecific values from total values. Specific binding was measured by incubating cells with ¹²⁵I-HDL (A, D) at 4 °C. To measure specific cell association, cells were incubated with ¹²⁵I-HDL (B, E) at 37 °C. For the measurement of transport, BAECs were cultured on inserts. The transport of ¹²⁵I-HDL (C, F) from the apical to basolateral compartment was measured at 37 °C. The results are presented as means ± SEM of three to six independent triplicate experiments (n=3). P was calculated by unpaired Mann Whitney U-test