Figure 2: Enhanced uptake of HDL from human APOM transgenic mice by bovine aortic endothelial cells (BAECs).

For fluorescence microscopy (A), BAECs were cultured until confluence on cover slips and incubated for 60min at 37° with 50 μg/ml 594Atto-HDL, fixed with 3.75% formaldehyde and counter-stained with DAPI for nuclear staining. (B) BAECs were incubated with ¹²⁵I-HDL of APOM transgenic mice at 37 °C for 1 hour in the absence (total) or in the presence of 40-fold excess of unlabeled HDL. Specific association was calculated by subtracting unspecific values from total values. The results are presented as means ± SEM of six independent triplicate experiments (n=3). P was calculated by unpaired Mann Whitney U-test.