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. Author manuscript; available in PMC: 2021 Dec 4.
Published in final edited form as: Sci Immunol. 2021 Jun 4;6(60):eabg1101. doi: 10.1126/sciimmunol.abg1101

Figure 5: Ggt5 and Abcc1 controlled homing of P2RY8+ lymphocytes to the BM.

Figure 5:

(a-d) Activated EV- or P2RY8-transduced polyclonal B cells were transferred into preimmunized mice. (a) GFP+ frequency in the BM divided by GFP+ frequency in the blood at 24hrs after cell transfer (n=7–10 per group). (b) Percentage of GFP+ cells in each mouse staining with CD45.2-PE injected intravascularly, divided by GFP- cell staining for the same (n= 3–10 per group). (c) GFP+ frequency in the BM divided by GFP+ frequency in the blood at 24hrs after cell transfer in Abcc1 chimeric mice. (d) Percentage of GFP+ cells in each individual Abcc1 chimeric mouse staining with CD45.2-PE injected intravascularly, divided by GFP- cell staining for the same (n= 3–7 per group in c,d). (e) GFP+ frequency of mature B, T and NK cells in the BM divided by GFP+ frequency in the blood in congenically marked mice reconstituted with EV- or P2RY8-transduced Abcc1+/+ or −/− BM. Data from Abcc1+/+ BM from this panel is also included in Fig 4d (n=6–7 per group). Data are pooled from seven (a), three (b,c,d), or two (e) experiments. P values determined by one-way ANOVA with Tukey’s multiple comparisons test (d). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Graphs depict mean with s.d. and points represent biological replicates.