Fig. 3. Both Rad27 and Msh2-Msh6 are required to form only mature repair product bands.

Assays of 5′ nick-directed repair of the CC substrate, in which different proteins were omitted or substituted as indicated. The amount of Rad27 used is indicated in pmole. All reactions also contain PCNA, RFC-Δ1N, and RPA. Other than Rad27, the amounts of all proteins used in the assays are as listed in the “Methods” section. MW, molecular weight markers. The percent repair corresponds to the fraction of all DNA in each individual lane susceptible to PstI cleavage, including those labeled “strand displacement” and “repair products”. Three independent experiments were performed.