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. 2021 Sep 22;12:5568. doi: 10.1038/s41467-021-25866-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative assay of 5′ nick-directed repair of the CC substrate in which different proteins were omitted or substituted as indicated. All reactions also contain PCNA, RFC-Δ1N, and RPA. Other than Rad27, the amounts of all proteins used in the assays are as listed in the “Methods” section. MW, molecular weight markers. The percent repair corresponds to the fraction of all DNA in each individual lane susceptible to PstI cleavage, including those labeled “strand displacement” and “repair products”. b The amount of repair for the reactions was quantified. Three independent experiments were performed and the average amount of repair observed is reported. Error bars represent the standard error of the mean, and individual values from the different experiments are indicated by the dots on each histogram bar. Bar graph was constructed in GraphPad Prism software.