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. 2021 Sep 22;12:5568. doi: 10.1038/s41467-021-25866-z

Fig. 5. Effect of substituting wild-type Msh2-Msh6 for two different mutant Msh2-Msh6 complexes when omitting Rad27.

Fig. 5

Representative assay of 5′ nick-directed repair of the CC substrate in which different proteins were omitted or substituted as indicated. All reactions also contain PCNA, RFC-Δ1N, and RPA. The amounts of all proteins used in the assays are as listed in the “Methods” section. MW, molecular weight markers. The percent repair corresponds to the fraction of all DNA in each individual lane susceptible to PstI cleavage, including those labeled “strand displacement” and “repair products”. Three independent experiments were performed.