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. 2021 Sep 22;11:18798. doi: 10.1038/s41598-021-97947-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Progressive outer nuclear layer (ONL) degeneration following retinal detachment. (A) Representative cryosection whole-retina tile image of a retinal detachment relative ONL thickness heatmap. Regions of interest used for analysis (dotted boxes), and retinal detachment height callipers (dashed arrows). (B) Height of retinal detachment at baseline. (C) Height of retinal detachment at day 14. (D) ONL cell count analysis. (E) ONL/INL thickness ratio analysis. Scale bar = 400 µm (A). *p < 0.05 vs. baseline day 0 between groups (n = 4–7).